Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/11326
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Immune recognition of the surface associated antigen, Tc-SAA-1, from infective larvae of Teladorsagia circumcincta
Authors: Nisbet, Alasdair
Knox, David
McNair, Carol
Meikle, Lynne
Smith, Stuart
Wildblood, Louise
Matthews, Jacquie
Contact Email: c.m.mcnair@stir.ac.uk
Keywords: immune response
surface associated antigen
stage-specific
Teladorsagia circumcincta
Issue Date: Jan-2009
Publisher: Blackwell Publishing
Citation: Nisbet A, Knox D, McNair C, Meikle L, Smith S, Wildblood L & Matthews J (2009) Immune recognition of the surface associated antigen, Tc-SAA-1, from infective larvae of Teladorsagia circumcincta, Parasite Immunology, 31 (1), pp. 32-40.
Abstract: A cDNA encoding a surface-associated antigen was amplified by reverse transcriptase polymerase chain reaction (PCR) from RNA extracted from Teladorsagia circumcincta exsheathed third stage larvae (xL3). The protein encoded by this cDNA, Tc-SAA-1, displays 77% identity over 162 amino acid residues to a surface associated antigen from Ancylostoma caninum (Ac-SAA-1). Antiserum raised against a bacterially-expressed recombinant form of Tc-SAA-1 reacted with a native protein in somatic and surface extracts of xL3 but not with L4 or adult parasites. Limited binding of anti-Tc-SAA-1 antibody was observed on the cuticular surface of xL3 s, however, regions of localization underlying the cuticle were observed. Incubation of xL3 T. circumcincta with anti-SAA rabbit serum failed to significantly inhibit penetration of the abomasal mucosa in vitro. IgA in abomasal mucus derived from sheep that had received a trickle infection of T. circumcincta bound recombinant Tc-SAA-1.
Type: Journal Article
URI: http://hdl.handle.net/1893/11326
DOI Link: http://dx.doi.org/10.1111/j.1365-3024.2008.01070.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: The Moredun Research Institute
The Moredun Research Institute
Aquaculture
The Moredun Research Institute
The Moredun Research Institute
The Moredun Research Institute
The Moredun Research Institute

Files in This Item:
File Description SizeFormat 
TcSAA.pdf244.37 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.