Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/1100
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Unrefereed
Title: Authenticating Production Origin of Gilthead Sea Bream (Sparus aurata) by Chemical and Isotopic Fingerprinting
Authors: Morrison, Douglas J
Preston, Tom
Bron, James
Henderson, R James
Strachan, Fiona
Bell, J Gordon
Cooper, Karen
Contact Email: j.e.bron@stir.ac.uk
Keywords: Fish nutrition
Nutrition
Mass spectrometry (MS)
Analytical
Issue Date: Jun-2007
Publisher: Springer / American Oil Chemists' Society (AOCS)
Citation: Morrison DJ, Preston T, Bron J, Henderson RJ, Strachan F, Bell JG & Cooper K (2007) Authenticating Production Origin of Gilthead Sea Bream (Sparus aurata) by Chemical and Isotopic Fingerprinting, Lipids, 42 (6), pp. 537-545.
Abstract: Recent EU legislation (EC/2065/2001) requires that fish products, of wild and farmed origin, must provide consumer information that describes geographical origin and production method. The aim of the present study was to establish methods that could reliably differentiate between wild and farmed European gilthead sea bream (Sparus aurata). The methods that were chosen were based on chemical and stable isotopic analysis of the readily accessible lipid fraction. This study examined fatty acid profiles by capillary gas chromatography and the isotopic composition of fish oil ( 13C, 18O), phospholipid choline nitrogen ( 15N) and compound specific analysis of fatty acids ( 13C) by isotope ratio mass spectroscopy as parameters that could reliably discriminate samples of wild and farmed sea bream. The sample set comprised of 15 farmed and 15 wild gilthead sea bream (Sparus aurata), obtained from Greece and Spain, respectively. Discrimination was achieved using fatty acid compositions, with linoleic acid (18:2n-6), arachidonic acid (20:4n-6), stearic acid (18:0), vaccenic acid (18:1n-7) and docosapentaenoic acid (22:5n-3) providing the highest contributions for discrimination. Principle components analysis of the data set highlighted good discrimination between wild and farmed fish. Factor 1 and factor 2 accounted for >70% of the variation in the data. The variables contributing to this discrimination were: the fatty acids 14:0, 16:0, 18:0, 18:1n-9, 18:1n-7, 22:1n-11, 18:2n-6 and 22:5n-3; 13C of the fatty acids 16:0, 18:0, 16:1n-7, 18:1n-9, 20:5n-3 and 22:6n-3; Bulk oil fraction 13C; glycerol/choline fraction bulk 13C; 15N; % N; % lipid.
Type: Journal Article
URI: http://hdl.handle.net/1893/1100
URL: http://www.springerlink.com/content/47378j3hj6v66u80/
DOI Link: http://dx.doi.org/10.1007/s11745-007-3055-3
Rights: Published in Lipids by Springer / American Oil Chemists' Society (AOCS). The original publication is available at www.springerlink.com
Affiliation: Scottish Universities Environmental Research Centre
Scottish Universities Environmental Research Centre
Aquaculture
University of Stirling
Aquaculture
Aquaculture
Scottish Universities Environmental Research Centre

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