Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/10473
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Ultrastructure and cytopathology of a rickettsia-like organism causing systemic infection in the redclaw crayfish, Cherax quadricarinatus (Crustacea: decapoda), in Ecuador
Authors: Romero, Xavier
Turnbull, James
Jimenez, Roberto
Contact Email: j.f.turnbull@stir.ac.uk
Keywords: Cherax quadricarinatus
rickettsia-like organisms
hemocytes
intracellular bacteria
fixed phagocytes
infection
Issue Date: Aug-2000
Publisher: Elsevier
Citation: Romero X, Turnbull J & Jimenez R (2000) Ultrastructure and cytopathology of a rickettsia-like organism causing systemic infection in the redclaw crayfish, Cherax quadricarinatus (Crustacea: decapoda), in Ecuador, Journal of Invertebrate Pathology, 76 (2), pp. 95-104.
Abstract: A study of the ultrastructural characteristics of an intracellular bacterium infecting the redclaw crayfish, Cherax quadricarinatus, a pathogen referred to previously as a rickettsia-like organism (RLO), revealed the presence of different developmental stages. These included a rod-shaped and uniformly electron-dense elementary body (EB) and an intermediate body (IB). The length of the EB varied between 0.48 and 0.6 microm, and the diameter was 0.3 microm. The IB was 0.75 to 1.1 microm long by 0.36 to 0.44 microm in diameter. Although the EB of this bacterium has ultrastructural characteristics similar to those of Rickettsiella, no information is available regarding its genetic relationship to this genus, and the intracellular bacterium should continue to be referred to as a rickettsia-like organism. The hemocytes had different levels of infection, and the RLO proliferated inside these cells. The EB appeared to be free in the cytoplasm of infected hemocytes and other cells; however, this might be a fixation artifact. The EB was also contained in membrane-bound vacuoles along with the IB. RLO colonies were observed inside small granular cells. No large granular cells were observed in the sections examined; therefore, no data were obtained regarding infection of this type of hemocyte. The fixed phagocytes on the external side of the terminal hepatic arterioles had an activated interrupted layer containing RLO bacteria. Stem cells in the hematopoietic tissue were also infected, and some cells were apparently being released into circulation.
Type: Journal Article
URI: http://hdl.handle.net/1893/10473
DOI Link: http://dx.doi.org/10.1006/jipa.2000.4952
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Stirling
Aquaculture
Acuatecnos CIA Ltd

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