|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Feeding-associated gene expression in sheep scab mites (Psoroptes ovis)|
|Citation:||McNair C, Billingsley P, Nisbet A & Knox D (2010) Feeding-associated gene expression in sheep scab mites (Psoroptes ovis), Veterinary Research, 41 (2).|
|Abstract:||The mite Psoroptes ovis is the causative agent of sheep scab. Although not usually fatal, the disease can spread rapidly and is a serious animal welfare concern. Vaccine development against ectoparasites has primarily focussed on two sources of candidate vaccine antigens - "exposed" antigens that are secreted in saliva during feeding on a host and "concealed" antigens that are usually expressed in the parasite gut and may be involved in digestion. Here, we sought to identify genes encoding proteins important for mite feeding and digestion by a subtractive suppressive hybridisation approach comparing mRNA transcript abundance in "fed" and "starved" mites. The study identified a variety of genes which are up-regulated by feeding mites. These included group 1, 5, 7 and 13 allergens including the previously described cysteine protease Pso o 1. In addition, numerous novel genes were identified here including some encoding potential salivary gland proteins and others encoding proteins which may facilitate feeding such as a serum opacity factor. An olfactory receptor-like protein was identified in the starved mite population which may help the mite to identify a host.|
|Rights:||Publisher is open-access. Open access publishing allows free access to and distribution of published articles where the author retains copyright of their work by employing a Creative Commons attribution licence. Proper attribution of authorship and correct citation details should be given.|
|Gene expression feeding.pdf||269.03 kB||Adobe PDF||View/Open|
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