Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/10330
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Vitamin C Enhances Vitamin E Status and Reduces Oxidative Stress Indicators in Sea Bass Larvae Fed High DHA Microdiets
Authors: Betancor, Monica
Caballero, Maria Jose
Terova, Genciana
Cora, Samuela
Saleh, Reda
Benitez-Santana, Tibiabin
Bell, J Gordon
Hernandez-Cruz, Carmen Maria
Izquierdo, Marisol S
Contact Email: m.b.betancor@stir.ac.uk
Keywords: DHA
Vitamin E
Vitamin C
Muscle morphology
Antioxidant enzymes
IGF
MyHC
Issue Date: Dec-2012
Publisher: Springer
Citation: Betancor M, Caballero MJ, Terova G, Cora S, Saleh R, Benitez-Santana T, Bell JG, Hernandez-Cruz CM & Izquierdo MS (2012) Vitamin C Enhances Vitamin E Status and Reduces Oxidative Stress Indicators in Sea Bass Larvae Fed High DHA Microdiets, Lipids, 47 (2), pp. 1193-1207.
Abstract: Docosahexaenoic acid (DHA) is an essential fatty acid necessary for many biochemical, cellular and physiological functions in fish. However, high dietary levels of DHA increase free radical injury in sea bass (Dicentrarchus labrax) larvae muscle, even when vitamin E (α-tocopherol, α-TOH) is increased. Therefore, the inclusion of other nutrients with complementary antioxidant functions, such as vitamin C (ascorbic acid, vitC), could further contribute to prevent these lesions. The objective of the present study was to determine the effect of vitC inclusion (3,600 mg/kg) in high DHA (5 % DW) and α-TOH (3,000 mg/kg) microdiets (diets 5/3,000 and 5/3,000 + vitC) in comparison to a control diet (1 % DHA DW and 1,500 mg/kg of α-TOH; diet 1/1,500) on sea bass larvae growth, survival, whole body biochemical composition and thiobarbituric acid reactive substances (TBARS) content, muscle morphology, skeletal deformities and antioxidant enzymes, insulin-like growth factors (IGFs) and myosin expression (MyHC). Larvae fed diet 1/1,500 showed the best performance in terms of total length, incidence of muscular lesions and ossification degree. IGFs gene expression was elevated in 5/3,000 diet larvae, suggesting an increased muscle mitogenesis that was confirmed by the increase in the mRNA copies of MyHC. vitC effectively controlled oxidative damages in muscle, increased α-TOH larval contents and reduced TBARS content and the occurrence of skull deformities. The results of the present study showed the antioxidant synergism between vitamins E and C when high contents of DHA are included in sea bass larvae diets.
Type: Journal Article
URI: http://hdl.handle.net/1893/10330
DOI Link: http://dx.doi.org/10.1007/s11745-012-3730-x
Rights: Publisher policy allows this work to be made available in this repository; The original publication is available at www.springerlink.com
Affiliation: Aquaculture
Universidad de Las Palmas de Gran Canaria
University of Insubria, Italy
University of Insubria, Italy
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria
Aquaculture
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria

Files in This Item:
File Description SizeFormat 
Lipids_Betancor.pdf1.39 MBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.