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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Production and propagation of fully inbred clonal lines in the Nile tilapia (Oreochromis niloticus L.)
Authors: Sarder, M Rafiq I
Penman, David
Myers, James M
McAndrew, Brendan
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Issue Date: 1-Nov-1999
Publisher: Wiley-Blackwell
Citation: Sarder MRI, Penman D, Myers JM & McAndrew B (1999) Production and propagation of fully inbred clonal lines in the Nile tilapia (Oreochromis niloticus L.), Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 284 (6), pp. 675-685.
Abstract: Fully inbred clonal lines of fish are likely to be of great value in research on immunology, sex determination, quantitative genetics, and toxicology. In this study on the Nile tilapia (Oreochromis niloticus), gynogenesis or androgenesis were used to produce a first generation of completely inbred fish, from which clonal lines were established using gynogenesis, androgenesis, hormonal sex reversal and intraline crosses. The clonal nature of these lines was verified by using multilocus DNA fingerprinting and the isozyme locus ADA*. Although these lines might be expected to be monosex in nature (all-female XX or all-male YY depending on the clone), one line did contain both sexes of fish. The presence of males in this gynogenetic clonal line and data from progeny testing of these males suggested that this line was homozygous for an allele or combination of alleles at an autosomal locus or loci which caused female to male sex reversal but with limited penetrance. Outbred clonal lines were also produced by crossing between different inbred clones.
Type: Journal Article
DOI Link:<675::AID-JEZ9>3.0.CO;2-D
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Bangladesh Agricultural University
National Marine Fisheries Service, USA

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