Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/10125
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Induction of diploid androgenetic and mitotic gynogenetic Nile tilapia (Oreochromis niloticus L.)
Author(s): Myers, James M
Penman, David
Basavaraju, Yaraguntappa
Powell, Stephen F
Baoprasertkul, Puttharat
Rana, Kausik
Bromage, Niall R
McAndrew, Brendan
Contact Email: b.j.mcandrew@stir.ac.uk
Keywords: Androgenesis
Gynogenesis
Tilapia Gene bank
Chromosome manipulation
Nile tilapia
Nile Tilapia
Issue Date: Feb-1995
Date Deposited: 9-Nov-2012
Citation: Myers JM, Penman D, Basavaraju Y, Powell SF, Baoprasertkul P, Rana K, Bromage NR & McAndrew B (1995) Induction of diploid androgenetic and mitotic gynogenetic Nile tilapia (Oreochromis niloticus L.). TAG Theoretical and Applied Genetics, 90 (2), pp. 205-210. https://doi.org/10.1007/BF00222203
Abstract: Androgenesis is a potentially valuable technique for recovering fish from gene banks composed of cryopreserved sperm, developing inbred lines, and analyzing patterns of inheritance. The procedure for producing diploid organisms whose nuclear DNA is wholly of paternal origin is dependent on: (1) the denucleation of "host" eggs, and (2) the inhibition of the first mitotic division in order to double the haploid sperm chromosome complement following fertilization of host eggs. Denucleation of tilapia (Oreochromis niloticus L.) eggs was carried out using UV irradiation. Treatment durations of 5-8 rain (total dose of 450-720 J/m 2) produced acceptable yields of viable denucleated eggs [22.9el.6% (eSE) of controls] as estimated by the survival of haploid androgenetic tilapia to 48 h post-fertilization. Successful mitotic inhibition was accomplished using a heat-shock of 42.5~ for 3-4 rain, applied at 2.5-min intervals from 22.5 to 30 min post-fertilization (mpf). The mean survival of androgenetic diploid fish to yolk-sac absorption for treatment groups varied from 0.4% to 5.3%, relative to the controls. Differences in the suceptibility of eggs from different females to UV irradiation were a significant factor in the overall yield of androgenetic diploids. Paternal effects did not significantly influence the androgenetic yield, suggesting that individual males would not be selected against. For comparative purposes mitotic gynogenetic "mitogyne" diploids were produced from UV-irradiated sperm. Mean survival to yolk-sac absorption varied from 0.5% to 10.64%, relative to controls. Similar optima for androgenetic and gynogenetic induction were found in the period 25-27.5 mpf (minutes post-fertilization). Induction treatments would appear to be operating on the same developmental events in both these techniques, and the results suggest that the UV irradiations used do relatively little damage to the eggs beyond nuclear inactivation. The results indicate that the production of androgenetic O. niloticus is possible on a consistent basis and that the application of this technique may be useful in quantitative and conservation genetics.
DOI Link: 10.1007/BF00222203
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Licence URL(s): http://www.rioxx.net/licenses/under-embargo-all-rights-reserved

Files in This Item:
File Description SizeFormat 
MyersEtal_TAG_1995.pdfFulltext - Published Version710.14 kBAdobe PDFUnder Embargo until 2999-12-29    Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.