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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Teleost fish spermatozoa contain a cytosolic protein factor that induces calcium release in sea urchin egg homogenates and triggers calcium oscillations when injected into mouse oocytes
Authors: Coward, Kevin
Campos-Mendoza, Antonio
Larman, Mark
Hibbitt, Olivia
McAndrew, Brendan
Bromage, Niall R
Parrington, John
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Keywords: fertilization
PLC xi
egg activation
Issue Date: 30-May-2003
Publisher: Elsevier
Citation: Coward K, Campos-Mendoza A, Larman M, Hibbitt O, McAndrew B, Bromage NR & Parrington J (2003) Teleost fish spermatozoa contain a cytosolic protein factor that induces calcium release in sea urchin egg homogenates and triggers calcium oscillations when injected into mouse oocytes, Biochemical and Biophysical Research Communications, 305 (2), pp. 299-304.
Abstract: Established studies in a variety of organisms including amphibians, fish, ascidians, nemerteans, echinoderms, mammals, and even a species of flowering plant, clearly demonstrate that an increase in intracellular egg calcium is crucial to the process of egg activation at fertilization. In echinoderms, egg activation appears to involve an egg phospholipase C gamma (PLCγ). However, numerous studies in mammalian species suggest that calcium is released from internal egg stores at fertilization by a sperm-derived cytosolic protein factor. Recent studies in the mouse have identified this sperm-derived factor as being a novel sperm-specific PLC isoform with distinctive properties (PLCζ). Homologues of PLCζ have since been isolated from human and cynomolgus monkey sperm. In addition, sperm factor activity has been detected in non-mammalian species such as chicken, Xenopus, and a flowering plant. Here we report evidence for the existence of a similar sperm-derived factor in a commercially important species of teleost fish, the Nile tilapia Oreochromis niloticus (L). Using an established bioassay for calcium release, the sea urchin egg homogenate, we demonstrate that protein extracts obtained from tilapia spermatozoa exhibit PLC activity similar to that seen in mammalian sperm extracts, and also induce calcium release when added directly to the homogenate. Further, tilapia sperm extracts induced calcium oscillations when injected into mouse oocytes.
Type: Journal Article
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Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Notes: Fishes Spermatozoa; Nile tilapia
Affiliation: University of Oxford
Michoacan University of San Nicolas de Hildalgo (UMSNH)
University College London
University of Oxford
University of Stirling
University of Oxford

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